金仙真
助理教授
哲学博士 (犹他大学)
理学学士 (俄亥俄州立大学)
金仙真教授于2011年获得俄亥俄州立大学药学科学学士学位,后在犹他大学获得药剂学与药物化学的博士学位,并在犹他大学肖恩·C·欧文教授的实验室进行博士后研究。博士和博士后研究期间,金仙真教授专注于工程化分裂荧光素酶,开发用于检测和定量不同形式癌症蛋白及其相互作用的检测方法,而后她将分裂荧光素酶技术的应用扩展到临床样本中监测抗体水平和测量免疫力。在完成博士后培训后,金仙真教授在加州大学旧金山分校(UCSF)医学系担任研究助理,研究HIV特别是研究物质或治疗组合对HIV转录水平的影响,利用各种转录组学和单细胞测序技术进行研究。金仙真教授的研究经历使她关注将分裂蛋白系统应用于新诊断和组学检测的设计与开发。她未来的研究兴趣将专注于工程化分裂蛋白系统,利用这些工具进行高通量检测和定量,以表征与致病途径和机制相关的生物分子。
Janssens J, Wedrychowski A, Kim SJ, Isbell C, Hoh R, Pillai S, Henrich T, Deeks SG, Roan N, Lee S, Yukl SA. Longitudinal changes in the transcriptionally active and intact HIV reservoir after starting ART during acute infection. J Virol. Accepted 11/25/2024.
Janssens J, Kim P, Kim SJ, Wedrychowski A, Kadiyala GN, Hunt PW, Deeks SG, Wong JK, Yukl SA. Mechanisms and efficacy of small molecule latency-promoting agents to inhibit HIV reactivation ex vivo. JCI Insight. 2024 Aug 20;9(19):3183084.
Kadiyala GN, Telwatte S, Wedrychowski A, Janssens J, Kim SJ, Kim P, Deeks S, Wong JK, Yukl SA. Differential susceptibility of cells infected with defective and intact HIV proviruses to killing by obatoclax and other small molecules. AIDS. 2024 Jul 15;38(9):1281-1291.
Beck S, Shin D, Kim SJ, Hedde PN, W Zhao. Digital protein detection in bulk solutions. ACS Omega. 2022 Oct 13;7(42):37714-37723.
Beck S, Nakajima R, Jasinskas A, Abram TJ, Kim SJ, Bigdeli N, Tifrea DF, Hernandez-Davies J, Davies DH, Hedde PN, Felgner PL, Zhao W. A protein microarray-based respiratory viral antigen testing platform for COVID-19 surveillance. Biomedicines. 2022 Sep 9; 10(9), 2238.
Kim SJ, Yao Z, Marsh MC, Eckert DM, Kay MS, Lyakisheva A, Pasic M, Bansal A, Birnboim C, Jha P, Galipeau Y, Langlois MA, Delgado JC, Elgort MG, Campbell RA, Middleton EA, Stagljar I, Owen SC. Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies. Nat Commun. 2022 Jul 1; 13(1)3716.
Kim SJ, Dixon AS, Owen SC. Split-enzyme immunoassay to monitor EGFR-HER2 heterodimerization on cell surfaces. Acta Biomater. 2021 Sep 5; S1742-7061(21)00587-0.
Kim SJ, Dixon AS, Adamovich PC, Robinson PD, Owen SC. Homogeneous immunoassay using a tri-part split-luciferase for rapid quantification of anti-TNF therapeutic antibodies. ACS Sens. 2021 May 28; 6(5):1807-1814.
Yao Z, Drecun L, Aboualizadeh F, Kim SJ, Li Z, Valcourt EJ, Manguiat K, Wood H, Plenderleith S, Yip L, Li X, Zhong Z, Yue FY, Snider J, Tomic J, Drebot MA, McGeer A, Ostrowski M, Mubareka S, Rini J, Owen S, Stagljar I. A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies. Nat. Commun. 2021 Mar 22;12(1):1806.
Kim SJ, Owen SC. Hyaluronic acid binding to CD44S is indiscriminate of molecular weight. Biochim Biophys Acta Biomembr. 2020 Sep 1;1862(9):183348.
Kim SJ, Hatch ST, Dixon AS, Owen SC. Split-enzyme fragment as a single affinity tag that enables protein expression, purification, and functional assays. Biotechnol Bioeng. 2019 Jul;116(7):1575-1583.
Taurin S, Almomen AA, Pollak T, Kim SJ, Maxwell J, Peterson CM, Owen SC, Janát-Amsbury MM. Thermosensitive hydrogels a versatile concept adapted to vaginal drug delivery. J Drug Target. 2018 Aug;26(7):533-550.
Dixon AS, Kim SJ, Baumgarter BK, Krippner S, Owen SC. A tri-part protein complementation system using antibody-small peptide fusions enables homogeneous immunoassays. Scientific Reports. Sci Rep. 2017 Aug 15;7(1):8186.
Park JW, Kim SJ, Kwag DS, Kim S, Park J, Youn YS, Bae YH, Lee ES. Multifunctional delivery systems for advanced oral uptake of peptide/protein drugs. Curr Pharm Des. 2015;21(22):3097-110.
Kwon JY, Lee KH, Cheon SH, Ryu HY, Kim SJ, Kim DI. Adsorptive loss of secreted recombinant proteins in transgenic rice cell suspension cultures. Plant Cell Reports. 2012 Mar;31(3):551-560.