Protein Identification
The facility provides reliable proteins identification from low-complexity (e.g. identification from gels, purified protein) as well as complex samples. Proteins are first proteolytically digested to peptides and then sequenced using a variety of MS/MS fragmentation techniques. Afterwards, peptides/proteins are identified by a Sequest (Proteome Discoverer) database search. To account for more complex samples or identifying hundreds of proteins present in cell line/tissue, different pre-fractionation methods and longer LC-gradients are used. Please contact us to discuss your MS-project in detail.
