讲座主题：Real-time quantitative detection of single sperm cell dynamics and photodamage using optical tweezers

日期：2024年9月20日（周五）

时间：15:30-16:30

地点：TD301

主讲人：周金华教授&钟振声教授

主持人：陈刚教授

关于主讲人：

周金华，湖南华容人，民主促进会会员，博士，教授，硕士生导师，现任安徽医科大学生物医学工程学院副院长，中国光学学会第三届生物医学光子学专业委员会，安徽省增材制造协会医疗分会副会长，中国生物医学工程学会资深会员，中国光学学会高级会员，《中国医学装备》杂志第四届和第五届编委，JBHI,OLEN,Opt.Lett,OE等十余种国内外期刊审稿人。

钟振声，广东东莞人，博士，硕士生导师，国家自然科学基金函评专家，现就职于安徽医科大学生物医学工程系。主要研究兴趣为（1）利用单细胞力学手段解决生物学及医学问题；（2）利用单分子力学手段研究核酸折叠机理，并开发基于复杂核酸折叠结构的生物检测技术。目前已主持并结题国自然青年基金等多个研究项目，发表SCI研究论文20余篇，并在Nature Chemical Biology杂志发表过学术评论文章。

讲座摘要：Male factors account for about half of all infertility cases, and sperm motility is an important criterion for the diagnosis of male infertility. Due to the high swimming speed of sperm, standard sperm motility analysis methods, such as computer-assisted sperm analysis, struggle to track the same sperm multiple times. On the other hand, to reduce procedure time and improve consistency and reproducibility, laser is widely proposed to be applied in assisted reproductive technologies, including sperm fixation, sperm selection, and intracytoplasmic sperm injection. However, quantitative studies on laser-induced photodamage of sperm are lacking. In this study, by using optical tweezers, we established a method to evaluate the mobility of human sperm. Our study revealed the dose effect of laser irradiation on photodamage of individual sperm cells, which provides an experimental basis for optimization of laser application in assisted reproductive technology to reduce photodamage-induced biosafety risks in the future. We proved that the real-time motility of sperm can be characterized by longitudinal rolling parameters using optical tweezers. Our study also revealed that as trapping time and laser power increase, the longitudinal rolling frequency of the optically trapped sperm decreases with an increasing percentage of pausing duration. This indicates the effect of laser power and duration on photodamage of individual sperm cells. This study provides a reference for future research on photodamage-prevention methods in laser-based sperm manipulation.

关键词：Optical tweezers; human sperm, photodamage; laser; assisted reproductive techniques