Unbiased proteomic studies can be performed using our high resolution Orbitrap mass spectrometers to provide accurate quantification data. The Facility offers several quantification strategies:

1. Label free quantification

Label-free quantification is a method in mass spectrometry that aims to determine the relative amount of proteins in two or more biological samples without any prior labeling. Comparing the ratio of ion abundances between identical peptides obtained in different experiment runs can be used to estimate differential expression. Label free quantification service is offered only in a collaborative setting.

 2. Labeling-based MS1 quantification (such as dimethyl and SILAC)

Metabolic labeling using SILAC

Stable isotope labeling using amino acids in cell culture (SILAC) is a highly accurate method to identify and quantify relative differential changes in complex protein samples under differential treatments. The SILAC method uses in vivo metabolic incorporation of isotopically labeled amino acids into proteins followed by mass spectrometry (MS). SILAC approaches are well suited for monitoring changes in post-translational modifications. The facility offers LC-MS/MS data acquisition and preliminary data analysis/quality control.

      3. TMT /  iTRAQ protein quantification

Isobaric labeling provides a fast and easy way for relative and absolute protein quantification with high throughput. The facility offers data acquisition and analysis/quality control. In case you would like to perform such experiments, we would be happy to assist in the planning phase.